NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link. Searched SlideID is highlighted in the table below.

Experiment:139
Title:AtGenExpress:Stress Treatments (Osmotic stress)
Date:2004-10-01
Description:AtGenExpress: A multinational coordinated effort to uncover the transcriptome of the multicellular model organism Arabidopsis thaliana. The activity of genes and their encoded products can be regulated in several ways, but transcription is the primary level, since all other modes of regulation (RNA splicing, RNA and protein stability, etc.) are dependent on a gene being transcribed in the first place. The importance of transcriptional regulation has been underscored by the recent flood of global expression analyses, which have confirmed that transcriptional co-regulation of genes that act together is the norm, not the exception. Moreover, many studies suggest that evolutionary change is driven in large part by modifications of transcriptional programs. An essential first step toward deciphering the transcriptional code is to determine the expression pattern of all genes. With this goal in mind, an international effort to develop a gene expression atlas of Arabidopsis has been underway since fall 2003. This project, dubbed AtGenExpress, is funded by the DFG, and will provide the Arabidopsis community with access to a large set of Affymetrix microarray data. As part of this collaboration, we have generated expression data from 80 biologicaly different samples in triplicate. Seeds of Arabidopsis thaliana Wild Type (col-0) were sown on rafts in Magenta boxes containing MS-Agar-media. After 2 days in the cold room (4°C, dark) the boxes were transferred to the long day chamber. Long day conditions were 16/8 hrs light/dark, 24°C, 50% humidity and 150 uEinstein/cm2 sec light intensity. At day 11 the rafts were transferred in Magenta boxes containing MS-liquid-media. At day 16 stress treatment started at 3 hrs of light period; samples taken at 0.5, 1, 3, 6, 12, 24 h after treatment (in selected indicated cases 0.25h and 4.0h too), control samples include 0h; roots and shoots were prepared separately; all treatments and preparations were done on the same batch of seedlings in one place (Lab J.Kudla/Ulm, Germany) by coworkers from the indicated groups.
ftp Link:ftp Link

Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File
AtGen_6-2111_Osmoticstress-Shoots-0.5h_Rep12266Shoots N10922111.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Osmotic stress (300 mM Mannitol), Group Kudla. Mannitol was added to a concentration of 300 mM in the Media. To add Mannitol the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added Mannitol. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-2112_Osmoticstress-Shoots-0.5h_Rep22267Shoots N10922112.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Osmotic stress (300 mM Mannitol), Group Kudla. Mannitol was added to a concentration of 300 mM in the Media. To add Mannitol the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added Mannitol. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-2121_Osmoticstress-Roots-0.5h_Rep12268Roots N10922121.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Osmotic stress (300 mM Mannitol), Group Kudla. Mannitol was added to a concentration of 300 mM in the Media. To add Mannitol the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added Mannitol. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-2122_Osmoticstress-Roots-0.5h_Rep22269Roots N10922122.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Osmotic stress (300 mM Mannitol), Group Kudla. Mannitol was added to a concentration of 300 mM in the Media. To add Mannitol the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added Mannitol. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-2211_Osmoticstress-Shoots-1.0h_Rep12270Shoots N10922211.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Osmotic stress (300 mM Mannitol), Group Kudla. Mannitol was added to a concentration of 300 mM in the Media. To add Mannitol the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added Mannitol. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-2212_Osmoticstress-Shoots-1.0h_Rep22271Shoots N10922212.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Osmotic stress (300 mM Mannitol), Group Kudla. Mannitol was added to a concentration of 300 mM in the Media. To add Mannitol the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added Mannitol. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-2221_Osmoticstress-Roots-1.0h_Rep12272Roots N10922221.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Osmotic stress (300 mM Mannitol), Group Kudla. Mannitol was added to a concentration of 300 mM in the Media. To add Mannitol the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added Mannitol. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-2222_Osmoticstress-Roots-1.0h_Rep22273Roots N10922222.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Osmotic stress (300 mM Mannitol), Group Kudla. Mannitol was added to a concentration of 300 mM in the Media. To add Mannitol the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added Mannitol. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-2311_Osmoticstress-Shoots-3.0h_Rep12274Shoots N10922311.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Osmotic stress (300 mM Mannitol), Group Kudla. Mannitol was added to a concentration of 300 mM in the Media. To add Mannitol the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added Mannitol. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-2312_Osmoticstress-Shoots-3.0h_Rep22275Shoots N10922312.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Osmotic stress (300 mM Mannitol), Group Kudla. Mannitol was added to a concentration of 300 mM in the Media. To add Mannitol the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added Mannitol. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-2321_Osmoticstress-Roots-3.0h_Rep12276Roots N10922321.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Osmotic stress (300 mM Mannitol), Group Kudla. Mannitol was added to a concentration of 300 mM in the Media. To add Mannitol the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added Mannitol. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-2322_Osmoticstress-Roots-3.0h_Rep22277Roots N10922322.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Osmotic stress (300 mM Mannitol), Group Kudla. Mannitol was added to a concentration of 300 mM in the Media. To add Mannitol the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added Mannitol. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-2411_Osmoticstress-Shoots-6.0h_Rep12278Shoots N10922411.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Osmotic stress (300 mM Mannitol), Group Kudla. Mannitol was added to a concentration of 300 mM in the Media. To add Mannitol the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added Mannitol. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-2412_Osmoticstress-Shoots-6.0h_Rep22279Shoots N10922412.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Osmotic stress (300 mM Mannitol), Group Kudla. Mannitol was added to a concentration of 300 mM in the Media. To add Mannitol the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added Mannitol. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-2421_Osmoticstress-Roots-6.0h_Rep12280Roots N10922421.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Osmotic stress (300 mM Mannitol), Group Kudla. Mannitol was added to a concentration of 300 mM in the Media. To add Mannitol the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added Mannitol. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-2422_Osmoticstress-Roots-6.0h_Rep22281Roots N10922422.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Osmotic stress (300 mM Mannitol), Group Kudla. Mannitol was added to a concentration of 300 mM in the Media. To add Mannitol the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added Mannitol. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-2511_Osmoticstress-Shoots-12.0h_Rep12282Shoots N10922511.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Osmotic stress (300 mM Mannitol), Group Kudla. Mannitol was added to a concentration of 300 mM in the Media. To add Mannitol the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added Mannitol. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-2512_Osmoticstress-Shoots-12.0h_Rep22283Shoots N10922512.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Osmotic stress (300 mM Mannitol), Group Kudla. Mannitol was added to a concentration of 300 mM in the Media. To add Mannitol the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added Mannitol. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-2521_Osmoticstress-Roots-12.0h_Rep12284Roots N10922521.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Osmotic stress (300 mM Mannitol), Group Kudla. Mannitol was added to a concentration of 300 mM in the Media. To add Mannitol the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added Mannitol. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-2522_Osmoticstress-Roots-12.0h_Rep22285Roots N10922522.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Osmotic stress (300 mM Mannitol), Group Kudla. Mannitol was added to a concentration of 300 mM in the Media. To add Mannitol the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added Mannitol. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-2611_Osmoticstress-Shoots-24.0h_Rep12286Shoots N10922611.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Osmotic stress (300 mM Mannitol), Group Kudla. Mannitol was added to a concentration of 300 mM in the Media. To add Mannitol the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added Mannitol. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-2612_Osmoticstress-Shoots-24.0h_Rep22287Shoots N10922612.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Osmotic stress (300 mM Mannitol), Group Kudla. Mannitol was added to a concentration of 300 mM in the Media. To add Mannitol the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added Mannitol. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-2621_Osmoticstress-Roots-24.0h_Rep12288Roots N10922621.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Osmotic stress (300 mM Mannitol), Group Kudla. Mannitol was added to a concentration of 300 mM in the Media. To add Mannitol the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added Mannitol. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-2622_Osmoticstress-Roots-24.0h_Rep22289Roots N10922622.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Osmotic stress (300 mM Mannitol), Group Kudla. Mannitol was added to a concentration of 300 mM in the Media. To add Mannitol the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added Mannitol. After the rafts were put back in the boxes, they were transferred back to the climate chamber.