NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link. Searched SlideID is highlighted in the table below.

Experiment:140
Title:AtGenExpress:Stress Treatments (Salt stress)
Date:2004-10-01
Description:AtGenExpress: A multinational coordinated effort to uncover the transcriptome of the multicellular model organism Arabidopsis thaliana The activity of genes and their encoded products can be regulated in several ways, but transcription is the primary level, since all other modes of regulation (RNA splicing, RNA and protein stability, etc.) are dependent on a gene being transcribed in the first place. The importance of transcriptional regulation has been underscored by the recent flood of global expression analyses, which have confirmed that transcriptional co-regulation of genes that act together is the norm, not the exception. Moreover, many studies suggest that evolutionary change is driven in large part by modifications of transcriptional programs. An essential first step toward deciphering the transcriptional code is to determine the expression pattern of all genes. With this goal in mind, an international effort to develop a gene expression atlas of Arabidopsis has been underway since fall 2003. This project, dubbed AtGenExpress, is funded by the DFG, and will provide the Arabidopsis community with access to a large set of Affymetrix microarray data. As part of this collaboration, we have generated expression data from 80 biologicaly different samples in triplicate. Seeds of Arabidopsis thaliana Wild Type (col-0) were sown on rafts in Magenta boxes containing MS-Agar-media. After 2 days in the cold room (4°C, dark) the boxes were transferred to the long day chamber. Long day conditions were 16/8 hrs light/dark, 24°C, 50% humidity and 150 µEinstein/cm2 sec light intensity. At day 11 the rafts were transferred in Magenta boxes containing MS-liquid-media. At day 16 stress treatment started at 3 hrs of light period; samples taken at 0.5, 1, 3, 6, 12, 24 h after treatment (in selected indicated cases 0.25h and 4.0h too), control samples include 0h; roots and shoots were prepared separately; all treatments and preparations were done on the same batch of seedlings in one place (Lab J.Kudla/Ulm, Germany) by coworkers from the indicated groups.
ftp Link:ftp Link

Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File
AtGen_6-3111_Saltstress-Shoots-0.5h_Rep12290Shoots N10923111.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Salt stress (150 mM NaCl), Group Kudla. NaCl was added to a concentration of 150 mM in the Media. To add NaCl the raft was lifted out. A magnetic stir bar and a stirrer were used to mix the media and the added NaCl. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-3112_Saltstress-Shoots-0.5h_Rep22291Shoots N10923112.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Salt stress (150 mM NaCl), Group Kudla. NaCl was added to a concentration of 150 mM in the Media. To add NaCl the raft was lifted out. A magnetic stir bar and a stirrer were used to mix the media and the added NaCl. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-3121_Saltstress-Roots-0.5h_Rep12292Roots N10923121.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Salt stress (150 mM NaCl), Group Kudla. NaCl was added to a concentration of 150 mM in the Media. To add NaCl the raft was lifted out. A magnetic stir bar and a stirrer were used to mix the media and the added NaCl. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-3122_Saltstress-Roots-0.5h_Rep22293Roots N10923122.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Salt stress (150 mM NaCl), Group Kudla. NaCl was added to a concentration of 150 mM in the Media. To add NaCl the raft was lifted out. A magnetic stir bar and a stirrer were used to mix the media and the added NaCl. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-3211_Saltstress-Shoots-1.0h_Rep12294Shoots N10923211.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Salt stress (150 mM NaCl), Group Kudla. NaCl was added to a concentration of 150 mM in the Media. To add NaCl the raft was lifted out. A magnetic stir bar and a stirrer were used to mix the media and the added NaCl. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-3212_Saltstress-Shoots-1.0h_Rep22295Shoots N10923212.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Salt stress (150 mM NaCl), Group Kudla. NaCl was added to a concentration of 150 mM in the Media. To add NaCl the raft was lifted out. A magnetic stir bar and a stirrer were used to mix the media and the added NaCl. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-3221_Saltstress-Roots-1.0h_Rep12296Roots N10923221.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Salt stress (150 mM NaCl), Group Kudla. NaCl was added to a concentration of 150 mM in the Media. To add NaCl the raft was lifted out. A magnetic stir bar and a stirrer were used to mix the media and the added NaCl. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-3222_Saltstress-Roots-1.0h_Rep22297Roots N10923222.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Salt stress (150 mM NaCl), Group Kudla. NaCl was added to a concentration of 150 mM in the Media. To add NaCl the raft was lifted out. A magnetic stir bar and a stirrer were used to mix the media and the added NaCl. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-3311_Saltstress-Shoots-3.0h_Rep12298Shoots N10923311.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Salt stress (150 mM NaCl), Group Kudla. NaCl was added to a concentration of 150 mM in the Media. To add NaCl the raft was lifted out. A magnetic stir bar and a stirrer were used to mix the media and the added NaCl. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-3312_Saltstress-Shoots-3.0h_Rep22299Shoots N10923312.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Salt stress (150 mM NaCl), Group Kudla. NaCl was added to a concentration of 150 mM in the Media. To add NaCl the raft was lifted out. A magnetic stir bar and a stirrer were used to mix the media and the added NaCl. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-3321_Saltstress-Roots-3.0h_Rep12300Roots N10923321.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Salt stress (150 mM NaCl), Group Kudla. NaCl was added to a concentration of 150 mM in the Media. To add NaCl the raft was lifted out. A magnetic stir bar and a stirrer were used to mix the media and the added NaCl. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-3322_Saltstress-Roots-3.0h_Rep22301Roots N10923322.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Salt stress (150 mM NaCl), Group Kudla. NaCl was added to a concentration of 150 mM in the Media. To add NaCl the raft was lifted out. A magnetic stir bar and a stirrer were used to mix the media and the added NaCl. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-3411_Saltstress-Shoots-6.0h_Rep12302Shoots N10923411.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Salt stress (150 mM NaCl), Group Kudla. NaCl was added to a concentration of 150 mM in the Media. To add NaCl the raft was lifted out. A magnetic stir bar and a stirrer were used to mix the media and the added NaCl. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-3412_Saltstress-Shoots-6.0h_Rep22303Shoots N10923412.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Salt stress (150 mM NaCl), Group Kudla. NaCl was added to a concentration of 150 mM in the Media. To add NaCl the raft was lifted out. A magnetic stir bar and a stirrer were used to mix the media and the added NaCl. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-3421_Saltstress-Roots-6.0h_Rep12304Roots N10923421.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Salt stress (150 mM NaCl), Group Kudla. NaCl was added to a concentration of 150 mM in the Media. To add NaCl the raft was lifted out. A magnetic stir bar and a stirrer were used to mix the media and the added NaCl. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-3422_Saltstress-Roots-6.0h_Rep22305Roots N10923422.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Salt stress (150 mM NaCl), Group Kudla. NaCl was added to a concentration of 150 mM in the Media. To add NaCl the raft was lifted out. A magnetic stir bar and a stirrer were used to mix the media and the added NaCl. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-3511_Saltstress-Shoots-12.0h_Rep12306Shoots N10923511.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Salt stress (150 mM NaCl), Group Kudla. NaCl was added to a concentration of 150 mM in the Media. To add NaCl the raft was lifted out. A magnetic stir bar and a stirrer were used to mix the media and the added NaCl. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-3512_Saltstress-Shoots-12.0h_Rep22307Shoots N10923512.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Salt stress (150 mM NaCl), Group Kudla. NaCl was added to a concentration of 150 mM in the Media. To add NaCl the raft was lifted out. A magnetic stir bar and a stirrer were used to mix the media and the added NaCl. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-3521_Saltstress-Roots-12.0h_Rep12308Roots N10923521.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Salt stress (150 mM NaCl), Group Kudla. NaCl was added to a concentration of 150 mM in the Media. To add NaCl the raft was lifted out. A magnetic stir bar and a stirrer were used to mix the media and the added NaCl. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-3522_Saltstress-Roots-12.0h_Rep22309Roots N10923522.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Salt stress (150 mM NaCl), Group Kudla. NaCl was added to a concentration of 150 mM in the Media. To add NaCl the raft was lifted out. A magnetic stir bar and a stirrer were used to mix the media and the added NaCl. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-3611_Saltstress-Shoots-24.0h_Rep12310Shoots N10923611.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Salt stress (150 mM NaCl), Group Kudla. NaCl was added to a concentration of 150 mM in the Media. To add NaCl the raft was lifted out. A magnetic stir bar and a stirrer were used to mix the media and the added NaCl. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-3612_Saltstress-Shoots-24.0h_Rep22311Shoots N10923612.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Salt stress (150 mM NaCl), Group Kudla. NaCl was added to a concentration of 150 mM in the Media. To add NaCl the raft was lifted out. A magnetic stir bar and a stirrer were used to mix the media and the added NaCl. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-3621_Saltstress-Roots-24.0h_Rep12312Roots N10923621.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Salt stress (150 mM NaCl), Group Kudla. NaCl was added to a concentration of 150 mM in the Media. To add NaCl the raft was lifted out. A magnetic stir bar and a stirrer were used to mix the media and the added NaCl. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-3622_Saltstress-Roots-24.0h_Rep22313Roots N10923622.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Salt stress (150 mM NaCl), Group Kudla. NaCl was added to a concentration of 150 mM in the Media. To add NaCl the raft was lifted out. A magnetic stir bar and a stirrer were used to mix the media and the added NaCl. After the rafts were put back in the boxes, they were transferred back to the climate chamber.