NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link. Searched SlideID is highlighted in the table below.

Experiment:141
Title:AtGenExpress:Stress Treatments (Drought stress)
Date:2004-10-01
Description:AtGenExpress: A multinational coordinated effort to uncover the transcriptome of the multicellular model organism Arabidopsis thaliana. The activity of genes and their encoded products can be regulated in several ways, but transcription is the primary level, since all other modes of regulation (RNA splicing, RNA and protein stability, etc.) are dependent on a gene being transcribed in the first place. The importance of transcriptional regulation has been underscored by the recent flood of global expression analyses, which have confirmed that transcriptional co-regulation of genes that act together is the norm, not the exception. Moreover, many studies suggest that evolutionary change is driven in large part by modifications of transcriptional programs. An essential first step toward deciphering the transcriptional code is to determine the expression pattern of all genes. With this goal in mind, an international effort to develop a gene expression atlas of Arabidopsis has been underway since fall 2003. This project, dubbed AtGenExpress, is funded by the DFG, and will provide the Arabidopsis community with access to a large set of Affymetrix microarray data. As part of this collaboration, we have generated expression data from 80 biologicaly different samples in triplicate. Seeds of Arabidopsis thaliana Wilde Type (col-0) were sown on rafts in Magenta boxes containing MS-Agar-media. After 2 days in the cold room (4°C, dark) the boxes were transferred to the long day chamber. Long day conditions were 16/8 hrs light/dark, 24°C, 50% humidity and 150 µEinstein/cm2 sec light intensity. At day 11 the rafts were transferred in Magenta boxes containing MS-liquid-media. At day 16 stress treatment started at 3 hrs of light period; samples taken at 0.5, 1, 3, 6, 12, 24 h after treatment (in selected indicated cases 0.25h and 4.0h too), control samples include 0h; roots and shoots were prepared separately; all treatments and preparations were done on the same batch of seedlings in one place (Lab J.Kudla/Ulm, Germany) by coworkers from the indicated groups.
ftp Link:ftp Link

Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File
AtGen_6-4111_Droughtstress-Shoots-0.5h_Rep12318Shoots N10924111.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Drought stress, Group Kudla. The plants were stressed by 15 min. dry air stream (clean bench) until 10% loss of fresh weight; then incubation in closed vessels in the climate chamber.
AtGen_6-4112_Droughtstress-Shoots-0.5h_Rep22319Shoots N10924112.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Drought stress, Group Kudla. The plants were stressed by 15 min. dry air stream (clean bench) until 10% loss of fresh weight; then incubation in closed vessels in the climate chamber.
AtGen_6-4121_Droughtstress-Roots-0.5h_Rep12320Roots N10924121.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Drought stress, Group Kudla. The plants were stressed by 15 min. dry air stream (clean bench) until 10% loss of fresh weight; then incubation in closed vessels in the climate chamber.
AtGen_6-4122_Droughtstress-Roots-0.5h_Rep22321Roots N10924122.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Drought stress, Group Kudla. The plants were stressed by 15 min. dry air stream (clean bench) until 10% loss of fresh weight; then incubation in closed vessels in the climate chamber.
AtGen_6-4211_Droughtstress-Shoots-1.0h_Rep12322Shoots N10924211.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Drought stress, Group Kudla. The plants were stressed by 15 min. dry air stream (clean bench) until 10% loss of fresh weight; then incubation in closed vessels in the climate chamber.
AtGen_6-4212_Droughtstress-Shoots-1.0h_Rep22323Shoots N10924212.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Drought stress, Group Kudla. The plants were stressed by 15 min. dry air stream (clean bench) until 10% loss of fresh weight; then incubation in closed vessels in the climate chamber.
AtGen_6-4221_Droughtstress-Roots-1.0h_Rep12324Roots N10924221.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Drought stress, Group Kudla. The plants were stressed by 15 min. dry air stream (clean bench) until 10% loss of fresh weight; then incubation in closed vessels in the climate chamber.
AtGen_6-4222_Droughtstress-Roots-1.0h_Rep22325Roots N10924222.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Drought stress, Group Kudla. The plants were stressed by 15 min. dry air stream (clean bench) until 10% loss of fresh weight; then incubation in closed vessels in the climate chamber.
AtGen_6-4311_Droughtstress-Shoots-3.0h_Rep12326Shoots N10924311.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Drought stress, Group Kudla. The plants were stressed by 15 min. dry air stream (clean bench) until 10% loss of fresh weight; then incubation in closed vessels in the climate chamber.
AtGen_6-4312_Droughtstress-Shoots-3.0h_Rep22327Shoots N10924312.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Drought stress, Group Kudla. The plants were stressed by 15 min. dry air stream (clean bench) until 10% loss of fresh weight; then incubation in closed vessels in the climate chamber.
AtGen_6-4321_Droughtstress-Roots-3.0h_Rep12328Roots N10924321.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Drought stress, Group Kudla. The plants were stressed by 15 min. dry air stream (clean bench) until 10% loss of fresh weight; then incubation in closed vessels in the climate chamber.
AtGen_6-4322_Droughtstress-Roots-3.0h_Rep22329Roots N10924322.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Drought stress, Group Kudla. The plants were stressed by 15 min. dry air stream (clean bench) until 10% loss of fresh weight; then incubation in closed vessels in the climate chamber.
AtGen_6-4411_Droughtstress-Shoots-6.0h_Rep12330Shoots N10924411.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Drought stress, Group Kudla. The plants were stressed by 15 min. dry air stream (clean bench) until 10% loss of fresh weight; then incubation in closed vessels in the climate chamber.
AtGen_6-4412_Droughtstress-Shoots-6.0h_Rep22331Shoots N10924412.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Drought stress, Group Kudla. The plants were stressed by 15 min. dry air stream (clean bench) until 10% loss of fresh weight; then incubation in closed vessels in the climate chamber.
AtGen_6-4421_Droughtstress-Roots-6.0h_Rep12332Roots N10924421.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Drought stress, Group Kudla. The plants were stressed by 15 min. dry air stream (clean bench) until 10% loss of fresh weight; then incubation in closed vessels in the climate chamber.
AtGen_6-4422_Droughtstress-Roots-6.0h_Rep22333Roots N10924422.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Drought stress, Group Kudla. The plants were stressed by 15 min. dry air stream (clean bench) until 10% loss of fresh weight; then incubation in closed vessels in the climate chamber.
AtGen_6-4511_Droughtstress-Shoots-12.0h_Rep12334Shoots N10924511.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Drought stress, Group Kudla. The plants were stressed by 15 min. dry air stream (clean bench) until 10% loss of fresh weight; then incubation in closed vessels in the climate chamber.
AtGen_6-4512_Droughtstress-Shoots-12.0h_Rep22335Shoots N10924512.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Drought stress, Group Kudla. The plants were stressed by 15 min. dry air stream (clean bench) until 10% loss of fresh weight; then incubation in closed vessels in the climate chamber.
AtGen_6-4521_Droughtstress-Roots-12.0h_Rep12336Roots N10924521.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Drought stress, Group Kudla. The plants were stressed by 15 min. dry air stream (clean bench) until 10% loss of fresh weight; then incubation in closed vessels in the climate chamber.
AtGen_6-4522_Droughtstress-Roots-12.0h_Rep22337Roots N10924522.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Drought stress, Group Kudla. The plants were stressed by 15 min. dry air stream (clean bench) until 10% loss of fresh weight; then incubation in closed vessels in the climate chamber.
AtGen_6-4611_Droughtstress-Shoots-24.0h_Rep12338Shoots N10924611.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Drought stress, Group Kudla. The plants were stressed by 15 min. dry air stream (clean bench) until 10% loss of fresh weight; then incubation in closed vessels in the climate chamber.
AtGen_6-4612_Droughtstress-Shoots-24.0h_Rep22339Shoots N10924612.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Drought stress, Group Kudla. The plants were stressed by 15 min. dry air stream (clean bench) until 10% loss of fresh weight; then incubation in closed vessels in the climate chamber.
AtGen_6-4621_Droughtstress-Roots-24.0h_Rep12340Roots N10924621.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Drought stress, Group Kudla. The plants were stressed by 15 min. dry air stream (clean bench) until 10% loss of fresh weight; then incubation in closed vessels in the climate chamber.
AtGen_6-4622_Droughtstress-Roots-24.0h_Rep22341Roots N10924622.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Drought stress, Group Kudla. The plants were stressed by 15 min. dry air stream (clean bench) until 10% loss of fresh weight; then incubation in closed vessels in the climate chamber.
AtGen_6-4711_Droughtstress-Shoots-0.25h_Rep12314Shoots N10924711.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Drought stress, Group Kudla. The plants were stressed by 15 min. dry air stream (clean bench) until 10% loss of fresh weight; then incubation in closed vessels in the climate chamber.
AtGen_6-4712_Droughtstress-Shoots-0.25h_Rep22315Shoots N10924712.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Drought stress, Group Kudla. The plants were stressed by 15 min. dry air stream (clean bench) until 10% loss of fresh weight; then incubation in closed vessels in the climate chamber.
AtGen_6-4721_Droughtstress-Roots-0.25h_Rep12316Roots N10924721.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Drought stress, Group Kudla. The plants were stressed by 15 min. dry air stream (clean bench) until 10% loss of fresh weight; then incubation in closed vessels in the climate chamber.
AtGen_6-4722_Droughtstress-Roots-0.25h_Rep22317Roots N10924722.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Drought stress, Group Kudla. The plants were stressed by 15 min. dry air stream (clean bench) until 10% loss of fresh weight; then incubation in closed vessels in the climate chamber.