NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link. Searched SlideID is highlighted in the table below.

Experiment:142
Title:AtGenExpress:Stress Treatments (Genotoxic stress)
Date:2004-10-01
Description:AtGenExpress: A multinational coordinated effort to uncover the transcriptome of the multicellular model organism Arabidopsis thaliana. The activity of genes and their encoded products can be regulated in several ways, but transcription is the primary level, since all other modes of regulation (RNA splicing, RNA and protein stability, etc.) are dependent on a gene being transcribed in the first place. The importance of transcriptional regulation has been underscored by the recent flood of global expression analyses, which have confirmed that transcriptional co-regulation of genes that act together is the norm, not the exception. Moreover, many studies suggest that evolutionary change is driven in large part by modifications of transcriptional programs. An essential first step toward deciphering the transcriptional code is to determine the expression pattern of all genes. With this goal in mind, an international effort to develop a gene expression atlas of Arabidopsis has been underway since fall 2003. This project, dubbed AtGenExpress, is funded by the DFG, and will provide the Arabidopsis community with access to a large set of Affymetrix microarray data. As part of this collaboration, we have generated expression data from 80 biologicaly different samples in triplicate. Seeds of Arabidopsis thaliana Wild Type (col-0) were sown on rafts in Magenta boxes containing MS-Agar-media. After 2 days in the cold room (4°C, dark) the boxes were transferred to the long day chamber. Long day conditions were 16/8 hrs light/dark, 24°C, 50% humidity and 150 µEinstein/cm2 sec light intensity. At day 11 the rafts were transferred in Magenta boxes containing MS-liquid-media. At day 16 stress treatment started at 3 hrs of light period; samples taken at 0.5, 1, 3, 6, 12, 24 h after treatment (in selected indicated cases 0.25h and 4.0h too), control samples include 0h; roots and shoots were prepared separately; all treatments and preparations were done on the same batch of seedlings in one place (Lab J.Kudla/Ulm, Germany) by coworkers from the indicated groups.
ftp Link:ftp Link

Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File
AtGen_6-5111_Genotoxicstress-Shoots-0.5h_Rep12342Shoots N10925111.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Genotoxic stress (1.5µg/ml bleomycin + 22 µg/ml mitomycin), Group Puchta. Bleomycin + mitomycin were added to the indicated concentration in the Media. To add the reagents the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added reagents. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-5112_Genotoxicstress-Shoots-0.5h_Rep22343Shoots N10925112.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Genotoxic stress (1.5µg/ml bleomycin + 22 µg/ml mitomycin), Group Puchta. Bleomycin + mitomycin were added to the indicated concentration in the Media. To add the reagents the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added reagents. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-5121_Genotoxicstress-Roots-0.5h_Rep12344Roots N10925121.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Genotoxic stress (1.5µg/ml bleomycin + 22 µg/ml mitomycin), Group Puchta. Bleomycin + mitomycin were added to the indicated concentration in the Media. To add the reagents the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added reagents. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-5122_Genotoxicstress-Roots-0.5h_Rep22345Roots N10925122.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Genotoxic stress (1.5µg/ml bleomycin + 22 µg/ml mitomycin), Group Puchta. Bleomycin + mitomycin were added to the indicated concentration in the Media. To add the reagents the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added reagents. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-5211_Genotoxicstress-Shoots-1.0h_Rep12346Shoots N10925211.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Genotoxic stress (1.5µg/ml bleomycin + 22 µg/ml mitomycin), Group Puchta. Bleomycin + mitomycin were added to the indicated concentration in the Media. To add the reagents the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added reagents. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-5212_Genotoxicstress-Shoots-1.0h_Rep22347Shoots N10925212.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Genotoxic stress (1.5µg/ml bleomycin + 22 µg/ml mitomycin), Group Puchta. Bleomycin + mitomycin were added to the indicated concentration in the Media. To add the reagents the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added reagents. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-5221_Genotoxicstress-Roots-1.0h_Rep12348Roots N10925221.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Genotoxic stress (1.5µg/ml bleomycin + 22 µg/ml mitomycin), Group Puchta. Bleomycin + mitomycin were added to the indicated concentration in the Media. To add the reagents the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added reagents. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-5222_Genotoxicstress-Roots-1.0h_Rep22349Roots N10925222.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Genotoxic stress (1.5µg/ml bleomycin + 22 µg/ml mitomycin), Group Puchta. Bleomycin + mitomycin were added to the indicated concentration in the Media. To add the reagents the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added reagents. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-5311_Genotoxicstress-Shoots-3.0h_Rep12350Shoots N10925311.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Genotoxic stress (1.5µg/ml bleomycin + 22 µg/ml mitomycin), Group Puchta. Bleomycin + mitomycin were added to the indicated concentration in the Media. To add the reagents the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added reagents. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-5312_Genotoxicstress-Shoots-3.0h_Rep22351Shoots N10925312.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Genotoxic stress (1.5µg/ml bleomycin + 22 µg/ml mitomycin), Group Puchta. Bleomycin + mitomycin were added to the indicated concentration in the Media. To add the reagents the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added reagents. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-5321_Genotoxicstress-Roots-3.0h_Rep12352Roots N10925321.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Genotoxic stress (1.5µg/ml bleomycin + 22 µg/ml mitomycin), Group Puchta. Bleomycin + mitomycin were added to the indicated concentration in the Media. To add the reagents the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added reagents. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-5322_Genotoxicstress-Roots-3.0h_Rep22353Roots N10925322.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Genotoxic stress (1.5µg/ml bleomycin + 22 µg/ml mitomycin), Group Puchta. Bleomycin + mitomycin were added to the indicated concentration in the Media. To add the reagents the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added reagents. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-5411_Genotoxicstress-Shoots-6.0h_Rep12354Shoots N10925411.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Genotoxic stress (1.5µg/ml bleomycin + 22 µg/ml mitomycin), Group Puchta. Bleomycin + mitomycin were added to the indicated concentration in the Media. To add the reagents the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added reagents. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-5412_Genotoxicstress-Shoots-6.0h_Rep22355Shoots N10925412.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Genotoxic stress (1.5µg/ml bleomycin + 22 µg/ml mitomycin), Group Puchta. Bleomycin + mitomycin were added to the indicated concentration in the Media. To add the reagents the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added reagents. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-5421_Genotoxicstress-Roots-6.0h_Rep12356Roots N1092AtGen_6-5421_Genotoxicstress-Roots-6.0h_Rep1-R.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Genotoxic stress (1.5µg/ml bleomycin + 22 µg/ml mitomycin), Group Puchta. Bleomycin + mitomycin were added to the indicated concentration in the Media. To add the reagents the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added reagents. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-5422_Genotoxicstress-Roots-6.0h_Rep22357Roots N10925422.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Genotoxic stress (1.5µg/ml bleomycin + 22 µg/ml mitomycin), Group Puchta. Bleomycin + mitomycin were added to the indicated concentration in the Media. To add the reagents the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added reagents. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-5511_Genotoxicstress-Shoots-12.0h_Rep12358Shoots N10925511.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Genotoxic stress (1.5µg/ml bleomycin + 22 µg/ml mitomycin), Group Puchta. Bleomycin + mitomycin were added to the indicated concentration in the Media. To add the reagents the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added reagents. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-5512_Genotoxicstress-Shoots-12.0h_Rep22359Shoots N10925512.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Genotoxic stress (1.5µg/ml bleomycin + 22 µg/ml mitomycin), Group Puchta. Bleomycin + mitomycin were added to the indicated concentration in the Media. To add the reagents the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added reagents. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-5521_Genotoxicstress-Roots-12.0h_Rep12360Roots N10925521.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Genotoxic stress (1.5µg/ml bleomycin + 22 µg/ml mitomycin), Group Puchta. Bleomycin + mitomycin were added to the indicated concentration in the Media. To add the reagents the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added reagents. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-5522_Genotoxicstress-Roots-12.0h_Rep22361Roots N10925522.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Genotoxic stress (1.5µg/ml bleomycin + 22 µg/ml mitomycin), Group Puchta. Bleomycin + mitomycin were added to the indicated concentration in the Media. To add the reagents the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added reagents. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-5611_Genotoxicstress-Shoots-24.0h_Rep12362Shoots N10925611.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Genotoxic stress (1.5µg/ml bleomycin + 22 µg/ml mitomycin), Group Puchta. Bleomycin + mitomycin were added to the indicated concentration in the Media. To add the reagents the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added reagents. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-5612_Genotoxicstress-Shoots-24.0h_Rep22363Shoots N10925612.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Genotoxic stress (1.5µg/ml bleomycin + 22 µg/ml mitomycin), Group Puchta. Bleomycin + mitomycin were added to the indicated concentration in the Media. To add the reagents the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added reagents. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-5621_Genotoxicstress-Roots-24.0h_Rep12364Roots N10925621.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Genotoxic stress (1.5µg/ml bleomycin + 22 µg/ml mitomycin), Group Puchta. Bleomycin + mitomycin were added to the indicated concentration in the Media. To add the reagents the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added reagents. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-5622_Genotoxicstress-Roots-24.0h_Rep22365Roots N10925622.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Genotoxic stress (1.5µg/ml bleomycin + 22 µg/ml mitomycin), Group Puchta. Bleomycin + mitomycin were added to the indicated concentration in the Media. To add the reagents the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added reagents. After the rafts were put back in the boxes, they were transferred back to the climate chamber.